Validation of antibody specificity using RNA Interference
نویسندگان
چکیده
منابع مشابه
Validation of a commercially available anti-REDD1 antibody using RNA interference and REDD1-/- mouse embryonic fibroblasts
REDD1 is a transcriptional target gene of p53 and HIF-1, and an inhibitor of mTOR (mechanistic target of rapamycin) complex 1 (mTORC1)-signaling through PP2A-dependent interaction, making it an important convergence point of both tumor suppression and cell growth pathways. In accordance with this positioning, REDD1 levels are transcriptionally upregulated in response to a variety of cellular st...
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Gene knockdown by RNA interference (RNAi) in Caenorhabditis elegans is readily achieved by feeding bacteria expressing double-stranded RNA (dsRNA). Enhanced RNAi (Eri) mutants facilitate RNAi due to their hypersensitivity to dsRNA. Here, we compare eight Eri mutants for sensitivity to ingested dsRNA, targeting a variety of tissue-specific genes.
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* Author for correspondence: Apekshita Singh, Amity Institute of Biotechnology, Amity University, Sector-125, Noida-201303 (UP), India. Email: [email protected]. Abstract. Metabolic engineering, which depends upon metabolite profiling and analysis, holds great potential, in combination with genomics, transcriptomics and proteomics, in facilitating the development of newly emerging technologies s...
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ژورنال
عنوان ژورنال: The FASEB Journal
سال: 2008
ISSN: 0892-6638,1530-6860
DOI: 10.1096/fasebj.22.1_supplement.1070.25